A signal conversion system using binding-induced strand displacement for disease biomarker assay

Using the principle of binding-induced DNA strand displacement (BINSD), a DNAzyme-powered nanomachine biosensor for multiple biomarkers via magnetic beads-based signal conversion was designed. It can convert multiple biomarkers recognition into the release of predesigned output nucleic acids tagged with the streptavidin proteins (SA-DNA) for activation of DNA nanomachines. In general, we adopted the complementary base pairing rules and affinity ligand specific recognition, and three types of signal conversion systems were constructed, respectively. They realized the universal, sensitive, accurate and specific detection of multiple biomarkers. Taking the advantage of strong anti-interference capability of the magnetic separation, this strategy could be employed for the detection of various biomarkers in clinical practice.