Identifying of Bt transgenic corn plants by PCR technology

Using fast,small-scale DNA extraction and PCR, 84 F2 transgenic Bt progeny were assayed. The results showed that 74 plants had Bt, 11 plants didn't. These plants were transplanted into field from greenhouse. After harvest, four lines were chosen to PCR analysis. The Bt separation in four lines was in conformity with Mendel's principle. PCR technology was reliable for identifying transgenic plants.