Studies of conditions which induce bone marrow stromal cells to differentiate into neural cells in vitro

ObjectiveTo explore the conditions to induce differentiation of BMSCs (bone marrow stromal cells)into NSCs(neural stem cells) and mature neural cells. MethodsThe effects of serum, RA, GDNF,BDNF and 2-ME at different concentration were observed. BMSCs were obtained from the marrow of monkey calf and cultured in NSCs culture medium, Nestin, CD133, NSE,β-tublin and GFAP were added as makers to identify the antigen character of cells, and patch-clamp was used to detect the electrophysiological properties of differentiated neural cells. ResultsIt was showed that most cells express the antigen of Nestin and CD133 on 8th day. neuron-like cells can be found after 3 days of induction which have the electrophysiological properties similar to neural cells, RA+GDNF with low concentration of serum was the most effective conditions as compared to other induction conditions in vitro. ConclusionThese results suggested that BMSCs could differentiate into mature neural cells efficiently in vitro in conditions of combining RA+GDNF with low concentration of serum.