Anti-proliferative applications of laccase immobilized on super-magnetic chitosan-functionalized halloysite nanotubes

Abstract Owing to the ubiquitous availability and simple biocatalysis, the anti-proliferative laccase holds enormous opportunities for anti-cancer applications. However, accessing efficient and specific (super-magnetically targetable) new delivery system for anti-proliferative laccase is vital step towards laccase based anti-cancer approach. Therefore, in this investigation, super-magnetized (Fe 3 O 4 ) and chitosan (CS) functionalized halloysite nanotubes (HNTs) (termed as Fe 3 O 4 -HNTs-CS) was facile synthesized. Further, laccase from Trametes versicolor was immobilized on Fe 3 O 4 -HNTs-CS (termed as Fe 3 O 4 -HNTs-CS- Lac ). Then free laccase and Fe 3 O 4 -HNTs-CS- Lac were evaluated for anti-proliferative properties against cancer cell lines of liver (HepG2), lung (H460), cervix (Hela) and stomach (AGS). Laccase and Fe 3 O 4 -HNTs-CS- Lac gave significant cytotoxicity against all studied cancer cell lines. Moreover, the apoptosis analysis and FE-SEM morphology observations of cells support the anti-proliferative potential of laccase immobilized on Fe 3 O 4 -HNTs-CS. Therefore, investigated Fe 3 O 4 -HNTs-CS- Lac is natural and super-magnetic nano-biocatalyst, having the significant anti-proliferative potential and furthermore, Fe 3 O 4 -HNTs-CS can be used as efficient and specific delivery system for other anti-cancer enzymes.