Expressing a GroEL gene in the different promoters of tobacco plants conferring resistance to tomato yellow leaf curl virus.

With the purpose of enhancing the resistance against tomato yellow leaf curl virus(TYLCV),the GroEL gene which was driven by CaMV35S,SUC2,PGHNBS promoters was exploited from Bemisia tabaci.Three plant expression vectors(P-GroEL,P-SUC2-GroEL,P-PGHNBS-GroEL) were successfully constructed and their transgenic tobacco plants were obtained by agrobacterium-mediated transformation,respectively.The result of RT-PCR proved that GroEL gene was expressed in three regenerated kanamycin resistant tobacco plants.The detection of virus infection showed that 19 resistant tobacco plants were acquired.Seven plants were transformed with expression vector P-GroEL,and P-PGHNBS-GroEL and P-SUC2-GroEL had 6 resisitant tobacco plants respectively.Double injections showed that 7 resistant tobacco plants were acquired,only one of which was transformed with expression vector P-GroEL.However,three resisitant tobacco plants were transformed with expression vector P-PGHNBS-GroEL and P-SUC2-GroEL,respectively.Two inoculation experiments reflected that it was transgenic tobacco with PGHNBS and SUC2 promoters expressed higher resistance toward TYLCV.Accordingly,it is feasible to acquire anti-TYLCV tobacco by constructing the vector with phloem-specific promoters.