The Study of Stem Cells Transplantation for Treatment of post-Infarction Congestive Heart Failure

Objective To explore the effect of stem cells transplantation on heart function after myocardial infarction. Methods The abdominal hypodermic adipose tissue of canines was isolated,culture in vitro and marked by DAPI. Canines were randomly divided into tow groups, control group and ADMSCs implantation group, which received IMDM medium and ADMSCs which marked by DAPI. To test heart function change, UCG and haemodynamics were measured in each group at 4 week. 4 weeks later immunofluorescence was given observe the information of the transplanted cells survival and differentiation. After MI and cell transplantation for 4 weeks HE staining, immunohistochemical, capillary densities, CVF and apoptosis cell population studie. Results There was a large amount of mesenchymal stem cells in canines adipose tissue. Surviving ADMSCs were identified by DAPI positive spots in border of infarcted region and transdifferentiated into cardiomyocytes characterized with a positive cardiac phenotype(troponinT)4 weeks after transplantation. LVESV、 left ventricle ponderal index、CVF in noninfarction zone and apoptosis cell population of ADMSCs transplantation group were lower than control group, whereas LVEF、LVSP and +dp/dtmax、capillary densities and CVF in infarction zone were significantly higher. Conclusion ADMSCs transplantation can improve1 heart function after myocardial infarction in canines and the possible reason may be correlate with microenvironment and produce a marked effect by angiogenesis, inhibition apoptosis and reduction collogen deposition in noninfraction zone.