Setup and Optimization of Primer for Protease Gene of Human Immunodeficiency Virus-1 as Evaluation Drug Resistance Manner in Iranian Patients

Human immunodeficiency virus HIV is a problem of global health After the development of anti retroviral therapy the mortality and morbidity rate was reduced in whom was infected with HIV However because of many reasons such as virologic immunologic HAART regimen has a different result in HIV infected patients CD T Lymphocyte count and viral load test confirmed and showed that in some patients have treatment failure and need to analysis HIV drugs target sequence and review in the administration of HAART regimen There is some commercial kit for HIV genotyping Beside of these commercial kits ANRS designed and released primer sets for amplification and sequencing of the HIV genes such as Pol Env In the present study protease sequence in the blood samples of patients was amplified and investigated with in house developed primer These protease sequences were not amplified by ANRS primers