TRANSFER OF ATRAZINE-RESISTANT GENE FROM NIGHTSHADE TO SOYBEAN CHLOROPLAST GENOME AND ITS EXPRESSION IN TRANSGENIC PLANTS

By binding the 32KD protein of chloroplast thylakoid membrane, the triazine herbicides such as atrazine inhibit the electron transport from the primary stable electron acceptor to thesecondary electron acceptor of photosystem Ⅱ and disturb the process of photosynthesis.A number of researches about the psbA gene coding the 32KD protein have been reported, but until now transfer of the atrazine-resistant psbA gene into the plant chloroplast genome has not yet succeeded. We cloned a chloroplast DNA fragment containing the psbA gene from an atrazine-resistant biotype of nightshade (Solanum nigrum) and analyzed the