Liposomes and their uses including shRNA molecules against thymidylate synthase

It provides an in vivo method of delivery of shRNA targeting TS. And short hairpin RNA (shRNA) capable of suppressing the expression of thymidylate synthase by RNAi effects, comprising PEG-modified cationic liposome, an anti-tumor agent, said shRNA on the surface of the PEG-modified cationic liposome coupled and, and has an overhang of at least 2 bases at the 3 'end, the anti-tumor agent.