РОЛЬ МАКРОФАГОВ В РЕГУЛЯЦИИ ПРОЦЕССА МИГРАЦИИ ЕМОПОЭТИЧЕСКИХ СТВОЛОВЫХ КЛЕТОК В СИСТЕМЕ КОСТНЫЙ МОЗГ – ПЕРИФЕРИЧЕСКАЯ КРОВЬ

Mechanisms by which HSCs mobilize into damaged organs are currently under scrutiny. Macrophage role in these processes is investigated. In this study, we performed a flow cytometry analysis of CD117+CD38+ and CD117+CD90low HSCs quantity in murine peripheral blood and bone marrow after liver and kidney injury under stimulation of phagocyte mononuclear system by injection of tamerit. This study have demonstrated increased levels of CD117+CD38+ HSCs in bone marrow after partial hepatectomy, along with their migration to peripheral blood in response to tamerit injection. We also demonstrated that peripheral blood CD117+CD38+ HSCs levels were elevated after kidney injury. After partial hepatectomy, nochanges of CD117+CD90low HSCs quantity in investigated tissues were detected. We observed increased number of CD117+CD90low HSCs in murine blood following kidney injury. Thus, we observed different influence of macrophage stimulation on the quantity of CD117+CD38+ and CD117+CD90low cells. These data suggest that HSCs mobilization from the bone marrow to peripheral blood depends, at least in part, on phagocyte mononuclear system, and that macrophage stimulation is important for proliferation and migration of various HSCs populations following liver and kidney injury.