Protective effect of olanzapine on transfected N2a cells of Alzheimer's disease related genes

Objective:To investigate olanzapine′s protective effect on death of double transfected(Swedish APP gene and presenilin1 gene) N2a cells induced by β-amyloid peptide(Aβ).Methods:Double transfected N2a cells were treated with olanzapine or/and Aβ.The MTT assay was used to determine cell viability,BCA assay to determine the protein content of cells,and the(Enzyme)-Linked-Immuno-Sorbent Assay(ELISA) to determine the intracellular and extrocellular levels of Aβ produced by double transfected N2a cells.Results:The olanzapine had protective effect on transfected N2a cell death induced by Aβ.The extracellullar Aβ of cells treated with olanzapine decreased significantly comparing to the vehicle(P0.05);the intracellular Aβ did not change significantly(P0.05).Conclution:This data suggest that olanzapine can decrease the production of β-amyloid peptide 42 in double transfected N2a cells and clinically may be helpful in protecting neuron and slowing down the progression of AD by this means.