The zinc-finger transcription factor Hindsight regulates ovulation competency of Drosophila follicles

The release of an egg from the ovary of a female animal is a process known as ovulation. Animals as different as humans and fruit flies ovulate in largely similar ways. Yet the systems involved in controlling ovulation are still not well understood. An egg cell develops within a collection of cells that help the egg to form properly. Together, this unit is called a follicle. During ovulation, connections between the egg and the rest of the follicle break down and the egg is eventually ejected. Ovulation happens in response to a hormone signal from the brain. In humans, this hormone is called luteinizing hormone, whereas in flies it is called octopamine. Specialized protein molecules on the surface of the follicle cells receive these hormone signals, but can only cause ovulation in mature follicles. It was not clear what allows only mature follicles to ovulate. Deady et al. have now used the fruit fly Drosophila melanogaster to examine ovulation to identify how the process is controlled. The results showed that a protein called Hindsight primes follicle cells for ovulation. When a follicle reaches its final stage (called stage 14 in flies), the gene for Hindsight becomes active and produces the protein. This protein then activates other genes. One of the activated genes makes a protein that receives the hormone signal, while another makes a protein that breaks down follicle cells and allows the egg to be released. The findings of Deady et al. reveal that Hindsight is needed for ovulation in flies. Further experiments then showed that the gene for equivalent human protein can be transplanted into flies and can still prime follicles for ovulation. This indicates that the genes in humans and flies may perform the same tasks. Studying ovulation is an important part of understanding female fertility and could help scientists to understand more about human reproduction. These results may also lead to new contraceptives and improved approaches for treating infertility.