Synthesis of bipolar tetradentate ligand and determination of fructose 1,6-diphosphate by resonance light scattering of its supramolecular polymer

In this paper, a novel method was established for determination of fructose 1,6-diphosphate (F-1,6-BP) based on resonance light scattering (RLS), and a type of bipolar tetradentate schiff base H4L was synthesized. The study showed that under the optimization condition with the ligand concentration of 5 nmol mL−1, pH 6.5, the reaction temperature of 25 °C, the reaction time of 30 min, RLS intensity had good linearity with the concentration of F-1,6-BP within the range of 0.4–5.0 nmol mL−1, the limit of detection was 0.16 nmol mL−1 and the relative standard deviation (RSD) was 1.83%. In vitro feasibility study, four groups of medical injections and two groups of human urine were analyzed, the RSD of six parallel experiments was approximately 2%, and the recovery value changed from 98.0 to 102.5%. As shown herein, this method has high sensitivity, good precision and recovery.