Regeneration of atp by immobilized microbial cells and its utilization for the synthesis of ATP and CDP-Choline

A number of fermentative methods for the production of various important nucleotides from their precursors have been developed in Japan, and some of these nucleotides have been industrially produced for more than a decade. For the production of these nucleotides, required ATP is usually supplied to the fermentation media either exogenously or by the naturally occurring ATP regenerating system involved in microbial cells. In this paper, the authors describe an attempt to immobilize ATP-regenerating microbial cells and then utilize such immobilized cells for the continuous production of various nucleotides.