ADVERSE EFFECT OF DIAZEPAM ON CYTOGENETIC AND BIOCHEMICAL EFFECTS IN WHITE MICE FED DIET SUPPLEMENT WITH CHITOSAN

The objective of the study was to know the impact of diazepam on the cytogenetic (mitotic, blast index) and biochemical parameters. For this purpose 75 mice, both sexes, aged (7-8) weeks were randomly divided into 5 groups and treated as follows. The first group (n=15) was administrated orally with diazepam (0.6mg/kg B.W.) for a period of 8 weeks by stomach tubes. The second group (n=15) was administrated with diazepam as in the first group and fed a diet containing Chitosan 1gm/kg b.w. The third group (n=15) was immunized by the killed vaccine of Pasteurella multocida injected (0.1ml) I/P at a dose of 3×10 8 cfu/ml two doses at two weeks interval and administrated daily with diazepam for a period of eight weeks. The fourth group (n=15) was administrated as in the first group and vaccinated as in the third group and fed a diet containing Chitosan (1gm/kg). The fifth group (n=15) was administrated with given 0.25m l distill water and was considered as negative control. Biochemical tests (ALT and Cholesterol) were measured at 60 days. Cholesterol was measured 1 st