Protein kinase c independent restoration of specific immune responsiveness in common variable immunodeficiency

Abstract 7,8-Disubstituted guanine ribonucleosides represent a class of B lymphocyte agonists that utilize a protein kinase C-independent signaling pathway. These compounds provide an alternate T helper signal for B cells and enhance antigen-specific humoral responses in the murine model and in an IL-2-dependent human model in vitro . They effectively restore high level immune responses in a variety of murine models of immunodeficiency both in vivo and in vitro . In this study we examined the potential of these compounds to improve antibody responses generated by cultured cells from patients with common variable immunodeficiency (CVI). The inability to mount normal humoral responses to antigen was confirmed in nine patients with diagnosed CVI (CVI: 37 ± 16, normal 653 ± 116 plaque-forming cells (PFC/culture; P P