Fluorescence quantificational RT-PCR detection reagent for bubble cell stomatitis virus, and preparation method

This invention relates to biological agent designed and synthesized with vesicular stomatitis virus gene fragments as the target, especially to its testing agent and its preparation method. This invention selects the virus N gene conservative fragments as the target and applies primer Express software and primer prere 50 software to synchesize the primers and probes. The optimum pairing screening experiment is processed to the designed multipair primers and probes to get the most suitable primes and probes. The fluorescent quantitation test agent of the virus contains a pair of special primers and a special fluorescent probes length of the amplified target fragment is 97bp.