Increasing antigenicity of B16 melanoma cell fraction with microwave hyperthermia

An injection of a fraction of a fraction (C30 mw) obtained by centrifugation at 30 kg of homogenate of B16 melanoma cells, treated with microwave hyperthermia (2450 MHz, CW, 44°C, for 20 minutes), increases the survival time (p 95%) of B16 melanoma.This delay of 26 days between these two injections corresponds to the moment where the synthesis of immunoglobulins is increased in protected mice.The earliest death (p < 0.001) of animals injected with untreated tumoral cell fraction (C30), and that received a suspension of viable B16 melanoma cells (107) can be explained by an inhibition of humoral immunity system.