Preparation and purification of polyclonal antibody against chymopapain

Objective: To prepare and purify polyclonal antibody against chymopapain,and to make a foundation for estabvishing an immunossay for chymopapain.Methods: New Zealand rabbit was immunizeol with chymopapain.Antiserum was purified by Protein A and analyzed by ELISA.Results: The titer of the antiserum obtained in this experiment by ELISA was up to 1∶380000 and the purity was proved to be high by SDS-PAGE.