The AP2 transcription factors DORNROSCHEN and DORNROSCHEN-LIKE redundantly control Arabidopsis embryo patterning via interaction with PHAVOLUTA.
2007
DORNROSCHEN ( DRN ) (also known as ENHANCER OF
SHOOT REGENERATION1 ; ESR1 ) and DRN-LIKE ( DRNL ;
also known as ESR2 ) are two linked paralogues encoding AP2
domain-containing proteins. drn mutants show embryo cell patterning
defects and, similarly to drnl mutants, disrupt cotyledon development
at incomplete penetrance. drn drnl double mutants with weak or strong
drnl alleles show more highly penetrant and extreme phenotypes,
including a pin -like embryo without cotyledons, confirming a high
degree of functional redundancy for the two genes in embryo patterning.
Altered expression of PIN1::PIN1-GFP and DR5::GFP in drn mutant
embryos places DRN upstream of auxin transport and response. A yeast
two-hybrid screen with DRN followed by co-immunoprecipitation and bimolecular
fluorescence complementation revealed PHAVOLUTA (PHV) to be a protein
interaction partner in planta. drn phv double mutants show an
increased penetrance of embryo cell division defects. DRNL can also interact
with PHV and both DRN and DRNL can heterodimerise with additional members of
the class III HD-ZIP family, PHABULOSA, REVOLUTA, CORONA and ATHB8.
Interactions involve the PAS-like C-terminal regions of these proteins and the
DRN/DRNL AP2 domain.
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