Evaluation of [18F]DPA-716 in a rat model of neuroinflammation: Comparison with [11C]DPA-713 and [18F]DPA-714 radiotracer for the TSPO 18 kDa

85 Objectives To evaluate [18F]DPA-716 a new candidate radiotracer for TSPO 18 kDa, using an acute inflammatory rat model in comparison with two other pyrazolopyrimidines: [18F]DPA-714 and [11C]DPA-713. Methods The AMPA rat model was used to study in vitro and in vivo specific and non-specific binding using autoradiography and µPET imaging on a Concorde Focus P220 PET camera. Results Autoradiography demonstrated a high contrast in the lesion versus the contralateral side (p Conclusions [18F]DPA-716 is a promising radiotracer for imaging acute neuroinflammation in rat. The properties of this new pyrazolopyrimidine are undoubtedly superior to those described for [11C]PK11195, but not to those recorded for [18F]DPA-714 and [11C]DPA-713. Research Support Funded by the EU FP6 networks DiMI (LSHB-CT-2005-512146) EMIL(LSHC-CT-2004-503569), Canceropole IDF and Institut du Cancer