Method for preparing chitosan through fermentation and enzymatic hydrolysis

2013 
The invention discloses a method for preparing chitosan through fermentation and enzymatic hydrolysis. The method comprises the following steps: drying crab shell or shrimp shell, and crushing; fermenting; carrying out enzymatic hydrolysis calcium removal and protein removal; and carrying out enzymatic hydrolysis for acetyl removal, washing with water, drying, and crushing. Compared with the prior art, the above enzymatic hydrolysis method has the advantages of realization of calcium removal and protein removal in a same step, substantial improvement of the production efficiency, no use of a strong aid or a strong alkali, low energy consumption, no pollution and environmental protection. A free calcium solution obtained after the enzymatic hydrolysis can be recovered to prepare an organic calcium preparation, so all products can be reasonably used to generate substantial economic values.
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