Anti-senescence effect of Pandanus conoideus Lamk on ROS metabolism in human umbilical vein endothelial cells

This study aimed to investigate whether red fruit oil (RFO, Pandanus conoideus Lamk) protects against endothelial senescence induced by reactive oxygen species (ROS) metabolism. 10 μg/mL RFO showed antioxidant activity with 59.3-fold higher potency than 1 mg/mL ascorbic acid. RFO also protected the human umbilical endothelial cell (HUVEC) proliferation against 2 mM hydrogen peroxide (H 2 O 2 ) exposure. RFO inhibited the activation of nuclear factor kappa B (NFκB), and degraded endothelin (ET-1). To determine whether the anti-senescence effect of RFO was related to oxidative response genes, RT 2 -profiler PCR array was performed with 84 oxidative stress-related genes. RFO increased the levels of superoxide dismutase (SOD1), glutathione peroxidase 3 (GPx), peroxiredoxin (TPx), and other antioxidants such as sulfiredoxin 1. RFO decreased the ROS metabolism-related genes such as aldehyde oxidase 1 ( AOX1 ), neutrophil cytosolic factor 1 ( NCF1 ), NO synthase 2 ( NOS2 ), and mitochondrial inner membrane protein ( MPV17 ), and the oxidative stress responsive genes such as NADPH oxidase 4 ( NOX4 ), sirtuin 2 ( SIRT2 ), C-C motif chemokine ligand 5 ( CCL5 ), heat shock protein 70 ( HSPA1A ), dual specificity phosphatase 1 (PTPN16), lactoperoxidase (LPO), and thyroid peroxidase (TPO). These results suggested that RFO could be a candidate agent for the treatment of vascular senescence.