Effect of supplementation of lutein and DL-methionine on certain haemato-biochemical parameters and serum concentration of corticosteronein captive Golden pheasants (Chrysolophus pictus)

Twenty four male golden pheasants were divided into 4 groups of 6 each. First group basal diet contained no supplementary lutein or DL-methionine (M0C0). The diet of the birds in group M0C1 were supplemented with lutein (40 mg/kg DM) without supplementary DL methionine, whereas those in group M1C0 were supplemented only with DL-methionine (1.5g/ kg DM). Birds in group M1C1 were fed both the supplements. A feeding trial of 60 days duration was conducted. Heterophill counts was significantly lower (P<0.001) in groups M0C1 and M1C1 as compared to group M0C0 and M1C0 and lymphocyte count was higher (P<0.05) in lutein supplemented group as compared to non-lutein supplemented groups. The ratio of H: L was significantly higher (P<0.001) in lutein supplemented groups. Other haematological and serum biochemical parameters were not significantly different among the groups. Serum concentrations of corticosterone were lower (P<0.001) in groups M0C1 and M1C1 as compared to groups M0C0 and M1C0. It was concluded that supplementation of lutein (40mg/kg DM) but not DL- methionine (0.15% DM) improved the capability of the Golden pheasant to combat stress.