Detection of a Point Mutation Associated with High-Level Isoniazid Resistance in Mycobacterium tuberculosis by Using Real-Time PCR Technology with 3-Minor Groove Binder-DNA Probes
2003
A crystalline mixture solid composition which has almost no hygroscopicity, is easy to handle and dissolve and hardly worn by abrasion, and comprisesalpha-D-glucopyranosyl-1,1-mannitol andalpha-D-glucopyranosyl-1,6-sorbitol and may further comprisealpha-D-glucopyranosyl-1,1-sorbitol in a certain case is obtained in an extremely short period of time by a power-saving and labor-saving process with a small-scale apparatus.A composition which comprises 20 to 75 wt % of alpha-D-glucopyranosyl-1,1-mannitol, 23 to 70 wt % of alpha-D-glucopyranosyl-1,6-sorbitol and 2 to 25 wt % of alpha-D-glucopyranosyl-1,1-sorbitol and is in a crystalline mixture solid state or has a specific surface area of 0.07 to 0.1 m /g, and a composition which comprises 30 to 75 wt % of alpha-D-glucopyranosyl-l,1-mannitol, 25 to 70 wt % of alpha-D-glucopyranosyl-1,6-sorbitol and has a specific surface area of 0.07 to 0.1 m /g.The above compositions are produced by a process comprising supplying a raw material, which is obtained by optionally removing trehalulose from a mixture containing isomaltulose and trehalulose produced from cane sugar through an isomerization reaction and hydrogenating into a kneader having a thin and long cooling zone together with seed crystals and discharging the obtained composition from an outlet continuously.
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