Characterization of novel micro fluidic approaches for increased sensitivity of sensors measuring surface reactions

On the basis of a simulation model for lab-on-a-chip systems, the three approaches of sample loop, hydraulic focusing and dielectrophoretic focusing were investigated with a view to increasing sensitivity. The bonding rate can be increased significantly using sample loop and hydraulic focusing. Both approaches involve a considerable extension of the measurement period, though the target bonding rate can be achieved more quickly with sample loop than with hydraulic focusing. By using suitable analytes which can be deflected using dielectrophoresis, the bonding rate can be significantly increased without any extension of the measurement period.