Interleukin-1β induces corticotropin-releasing factor-41 release from cultured hypothalamic cells through protein kinase C and cAMP-dependent protein kinase pathways

Abstract Interleukin-1β (IL-1β) induces a dose-dependent increase in the release of corticotropin-releasing factor-41 (CRF) from dispersed rat fetal hypothalamic cells in culture. This release of CRF could be inhibited by the protein kinase C inhibitor H-7, and by the protein kinase A inhibitor IP-20. This suggests that both protein kinase C and protein kinase A-dependent pathways are involved in the response of CRF to IL-1β. Dexamethasone also blocked the CRF response to IL-1β, indicating that activated glucocorticoid receptors can inhibit the response of CRF to IL-1β.