Cu2+‐imprinted cross‐linked chitosan resin as micro‐column packing materials for online chemiluminescence determination of trace copper

The Cu2+-imprinted cross-linked chitosan resin was synthesized and the binding characteristic of the resin to Cu2+ was evaluated. The prepared resin was packed into a micro-glass column and used as micro-separating column. The micro-separating column was connected into the chemiluminescence flow system and placed in front of the window of the photomultiplier tube. Based on the luminol–hydrogen peroxide chemiluminescence system, a flow injection online chemiluminescence method for determination of trace copper was developed and trace Cu2+ in complex samples was successfully determined. The proposed method improved the shortcomings of chemiluminescence method's poor selectivity. Copyright © 2010 John Wiley & Sons, Ltd.