Hydrophobic interaction chromatography of Trichoderma reesei cellulases on polypropylene glycol–sepharose

The fractionation of Trichoderma reesei cellulases complex by hydrophobic interaction chromatography was investigated using a mild hydrophobic ligand—polypropylene glycol immobilized on Sepharose CL-6B. The influence of different salt type (sodium chloride, sodium sulfate, and ammonium sulfate) and their concentration in the mobile phase, on the chromatographic behavior of T. reesei cellulases was evaluated. A partial fractionation of β-glucosidase was obtained using 13 and 16% (w/v) sodium sulfate and ammonium sulfate in eluent buffer, respectively.