Cytotoxicity of Combination Chitosan with Different Molecular Weight and Ethanol Extracted Aloe vera using MTT Assay

The use of combination chitosan and ethanol extracted Aloe vera is considered to have activity to promote bone healing. The requirement dental material used in oral cavity include good biocompatibility, non toxic, non carcinogenic and nor allergenic response. The aim of this study was to examine the cytotoxicity combination chitosan with different molecular weight and ethanol extracted Aloe vera using MTT assay. 1 % chitosan gel (w/v) was made from chitosan powder with high (ChH) and low (ChL) molecular weight. 1 % chitosan gel was combined with ethanol extracted Aloe vera with concentration 50 % (Av1) and 75% (Av2). It divided five groups: control group, ChH-Av1, ChH-Av2, ChL-Av1 and ChL-Av2. Each of sample was immersed in eppendorf microtubes consist of media culture. After 24 hours, the immersion of media culture were used to investigate the cytotoxic effect to BHK-21 cell lines by MTT assay method. The density of optic formazan was indicated the number of living cells. All data were statistically analyzed by one-way Anova. The result showed that percentage of the living cells of all groups was uper 50 % using parameter CD50. There was no significant difference among treatment groups. It can be concluded that combination chitosan with different molecular weight and ethanol extracted Aloe vera were non toxic for BHK-21 cell culture.