Agrobacterium-mediated transformation of P12A* 3C gene of foot-and-mouth disease virus type O into maize.

The P12A3C gene of food-and-mouth disease virus(FMDV) type O was transformed into type Ⅱ callus maize inbred line 18-599 via Agrobacterium-mediated transformation.25 resistant transgenic plants were obtained after the primary screening on the medium containing hygromycin.The detection by PCR,PCR-southern and RT-PCR indicated that the P12A3C gene was integrated into the genome of 18-599 and expressed.The detection of T1 generation indicated that the P12A3C gene could be stably transmited to the next generation.These results provided experimental evidence for production of oral FMDV vaccine.