Abstract TMP60: RNA Sequencing Reveals Novel MicroRNA Profiles in Neural Progenitor Cells After Stroke

Background: Neurogenesis is associated with functional recovery after stroke. However, the underlying molecular mechanisms have not been fully investigated. We hypothesized that stroke changes miRNA profiles in neural progenitor cells (NPCs), which is closely associated with stroke-induced neurogenesis. Methods and Results: Using a novel cross-linking immunoprecipitation to crosslink Ago2-RNA complexes followed by RNA sequencing (Ago2 CLIP-seq) approach, we profiled the entire small RNA population in NPCs harvested from the non-ischemic and ischemic subventricular zone (SVZ) of ischemic animals (n=6/group). Ago2 CLIP-seq analysis showed that over 7 million reads were found in normal and ischemic NPC libraries. In non-ischemic NPCs, the top 10 highly enriched miRNAs were miR-26, -27b, -127, -99b, -191, -125b-5p, let-7f, let-7i, -541 and -151. In contrast, stroke significantly changed the top 10 enriched miRNAs. Bioinformatics analysis revealed that these miRNAs could target genes involved in cytoskeleton r...