Inhibitory effects of Emodin on cholangiocarcinoma QBC939 cells proliferation and mitogen-activated protein kinase signal pathway in vitro

Objective To investigate the effects of Emodin in growing inhibition and down-regulating mitogen-activated protein kinase (MAPK) in vitro.Methods QBC939 cells were cultured with Emodin for different concentrations of Emodin.The inhibition of cell proliferation was detected by methyl thiazol tetrazolium (MTT).The expressions of c-myc mRNA and protein were detected by reverse transcriptasepolymerase chain reaction (RT-PCR) and Western blotting,MAPK,p-MAPK was detected by Western blotting.Results Emodin inhibited cell proliferation of QBC939 cell.apoptosis rate of QBC939 cells treated with 0,20,40,80 μmol/L Emodin for48 h was (4.73 ±0.88)％,(21.27 ±4.04)％,(38.68 ±4.57) ％,(62.45 ± 6.12) ％ (P ＜ 0.05) Emodin could down-regulating c-myc,p-MAPK level (P ＜0.05).but MAPK expression level had no significant change.Conclusion Emodin can inhibite cell proliferation of human cholangiocarcinoma cell QBC939.The mechanism is associated with down-regulating MAPK. Key words: QBC939;  Emodin;  Mitogen-activated protein kinase