Study on the Impact of Fluorescence Labeling Reaction of Chitosan-calcium Alginate Microsphere on Microsphere Membrane Strength

This paper investigates the impact of fluorescence labeling reaction of chitosan-calcium alginate microsphere for labeling on microphere membrane strength in simulated body liquid under different conditions in order to detect such microspheres in vivo.With chitosan and sodium alginate as materials for preparing microsphere and with isothiocyanate FITC as fluorescence label for labeling microspheres,the rate of expansion of FITC-labeled chitosan-alginate microspheres is determined to characterize microsphere membrane strength.The FITC-labeled microspheres are prepared with the FITC-labeled chitosan of which concentration is 0.015 g/mL reacting 30 min with calcium alginate microspheres.On the other hand,chitosan molecular weight is 50 000,the degree of deacytylation is 85% and the solution concentration is 0.01 g/mL.Such FITC-labeled microspheres show better membrane strength and stability.The appropriate experimental conditions are provided to study the distribution、absorbency and biodegradation characteristics of drug-loaded chitosan-alginate microsphere in vivo.