[Expression, subcellular localization and nuclear translocation of transcription factor up stream stimulatory factor-1 in odontoblasts].

Objective To examine the expression and subcellular localization of transcription fact USF1 in odontoblasts and investigate whether nuclear translocation occurs under stimuli.Methol Odoutoblasts MDPC-23 were cultured on coverslips and divided into 2 groups.Group 1 received no stimul and group 2 was stimulated by nicotine with various concentrations respectively for 1 h.Then the mountings odoutoblasts were prepared and immunocytochemieal staining was performed with specific USF1 antibody v SABC method.Hela cells were used as positive control.Results The staining was positive in the cytoplas of odontoblasts in group 1,but in the nuclei of Hela cells and in 100 mg/L nicotine-stimulated odontoblas in group 2.Conclusions There exists USF1 protein in odontoblasts,which locates in the cytoplasm a cotdd translocate into nuclei under the stimulation of nicotine.