Hydrogen peroxide inhibits proliferation and endothelial differentiation of bone marrow stem cells independent of reactive oxygen species generation

abstract Article history:Received 29 May 2014Accepted 9 July 2014Available online 21 July 2014Keywords:Hydrogen peroxideOxidative stressROSOct-4Endothelial differentiationBone marrow stem cell Aims: The present study was to investigate the effect of hydrogen peroxide (H 2 O 2 )onbonemarrowstemcellsand their endothelial differentiation and the underlying mechanisms in vitro.Main methods: Rat bone marrow multipotent adult progenitor cells (MAPCs) were used as the source of bonemarrow stem cells, and treated with H 2 O 2 (with the final concentration from 0 to 50 μM) with or without N-acetylcysteine(NAC,0.1mM).Reactiveoxygenspecies(ROS)wasmeasuredbyelectronparamagneticresonance(EPR) and fluorescent microscope. Flow cytometry and immunoblotting were used to determine apoptosis anddifferentiation of MAPCs.Key findings: H 2 O generated a significant amount of intracellular and extracellular ROS in the culture system,substantially inhibited the proliferation of MAPCs and Oct-4 expression, and induced their apoptosis in a dose-dependent manner. Exposure to H