Effects of Astragalus membranaceus and Panax notoginseng on the transformation of bone marrow stem cells and proliferation of EPC in vitro

Objective: To investigate the effect and the possible mechanism underlying the promotional effect of Astragalus membranaceus and Panax notoginseng on the transformation of bone narrow stem cells and proliferation of EPC.Method: The marrow blood was collected in the patients with ischemia of lower limbs and BM-MNCs were separated and proliferated under different conditions.A.morphologic observation was performed and the ratio of CD~+_(34) cells was measured.Result: The shuttle shaped cells lined up as bunches with several round cells scattered.The ratio of CD~+_(34) cells was significantly increased in groups treated with medium(P0.01) and lower(P0.05) dosages of A.membranaceus and medium(P0.01) and high dosages(P0.01) of P.notoginseng respectively as compared with control group.Conclusion: A.membranaceus and P.notoginseng can promote the transformation and proliferation of EPC.