EXTRACTION AND PURIFICATION AND MOLECULAR EVALUATION OF STAPHYLOCOCCUS AUREUS PROTEIN A

Staphylococcus aureus is categorized at the same range of Streptococci and Listeria. Grampositive cocci, arranged in grape-like clusters in pairs and chains with non-motile tetrads, Aerobic, Catalase positive and oxidase-negative (OX-) so that there exist more than 47 species and subspecies of the genus Staphylococcus. This paper aims to extract and purify Staphylococcus aureus protein A. Staphylococcus aureus subsp. aureus (strain NCTC 8325) was used to extract protein A. The stages mentioned to extract protein A include three stages of break down cell walls of bacteria, its release from the bacterial wall and the last purification. The results from findings have shown that all the methods used to date found suitable to break and slip cell walls of bacteria where the amount of protein A released was substantial, yet it has to draw a particular attention that the most amount of protein gains from Lysozyme method.