Biodegredable Electrospun Material for Tissue Engineering

The PCL known for its slow biodegradability offers advantageous possibilities for its applications in the field of skin scaffold production. In the current study PCL mats with micro scaled structure were produced via electrospinning and treated with NaOH solution for enhanced cell adhesion. The scaffolds were evaluated as an efficient stem cell growing material by human skin keratinocytes and fibroblasts culture. The cell viability assay (CellTiter-Blue) of the as spun scaffolds was compared to previously prepared PU electrospun scaffolds, fibrin scaffolds and amniotic membrane scaffold. Significant difference of cell adhesion was evident between the natural and synthetic scaffolds, as for the electrospun scaffolds the significance between, as well as for the NaOH treated samples was less evident. The repetitiveness of the different donor cells fluorescence within the same scaffold material was also present.