Fabrication and characterization of water-dispersed chitosan nanofiber/poly(ethylene glycol) diacrylate/calcium phosphate-based porous composites

Abstract We evaluated the capacity of chitosan nanofiber (CNF)- and poly(ethylene glycol) (PEG)-based hydrogel/calcium phosphate hybrid (CNF-PEG/CaP) composites to act as scaffolding materials. CNF-PEG/CaP composites were fabricated by mineralization of CNF-PEG hydrogels using an alternate soaking method. The amount of CaP mineralized on CNF-PEG hydrogels increased as the ratio of CNF in the CNF-PEG hydrogel increased. Young’s modulus of CNF-PEG/CaP hydrogels was enhanced by increase in CNF ratio. It was further confirmed that osteoblasts embedded on the CNF-PEG/CaP composites were viable after incubation for 5 days and firmly attached to the CaP porous layer, forming an extensive cell-scaffold leading to cell–cell interactions. These results indicated that the micro-porous structure of CNF-PEG hydrogels is suitable for CaP to be utilized as a scaffold for bone regeneration.