Cloning and Bioinformatic Analysis of Wheat DHARGene

In the study,aDHAR(Dehydroascorbate reductase)gene of wheat was cloned and characterized using the electronic cloning,RT-PCR,and bioinformatics methods.The results showed that the DHAR gene cloned was 1 178 bp,encoding 263 amino acids with molecular weight of 289 08.2Da and PI of 6.84.It contained the GST-N family and the typical GST-CDHAR constructure domain,belonging to the thioredoxin superfamily and GST-C superfamily,respectively.The protein sequence had no signal peptide,belonged to a hydrophilic protein,and was located in the chloroplast.Its secondary structures were mainly the random coil(40.30%)and α-helix(37.64%)as well as a small amount of extended strands(17.11%).It had higher sequence similarities with those of other plants,including Hordeum vulgare,Brachypodium distachyon,Zea may,Oryza sativa,Arabidopsis thaliana and so on,among which its evolution distance was closest to that(BAJ97007.1)of Hordeum vulgare.