Delivery of Probiotic to Microbiome by Layer-by-Layer Encapsulation

The probiotic alginate microcapsules of a matrix type were obtained by extrusion method. The coating with an additional layer (layer-by-layer) was carried out by placing the alginate microcapsules with bacteria included in them in a 0.4% solution of chitosan or pullulan. Microcapsules look like spherical particles, white, with a smooth surface. The size of microcapsules is 102–145 microns. The efficiency of L. acidophilus AA-1 cells immobilization for uncoated (alginate) and coated with chitosan and pullulan microcapsules was 96.35±1.65; 95.28±2.31 and 94.43±2.31, respectively. The survival rate of strains encapsulated in alginate-chitosan and alginate-pullan under model gastrointestinal conditions in vitro increased on average by 50-70% compared with free cells.