High-performance liquid chromatographic separation of potato glycoalkaloids

Abstract Separations of three glycoalkaloids ( a -chaconine, β-chaconine, and a -solanine) have been achieved by using three different columns: μBondapak C 18 , μBondapak NH 2 , and a carbohydrate analysis column. These methods have been employed to examine the purity of the potato glycoalkaloids isolated by thin-layer and column chromatographic separations. Also, samples of tubers, peels, blossoms, and sprouts have been analyzed to determine their content of a -chaconine, β-chaconine, and a -solanine by using the carbohydrate and μBondapak NH 2 columns.