Cell Biosensor for Detection of Phenol in Aqueous Solutions

A microbial sensor for concentration measurement of phenol in aqueous solutions has been developed. Phenol-utilizing cellsPseudomonas putida GFS-8 immobilized in poly(vinyl)alcohol cryogel were used as a biological transducer. Relationships between phenol concentration in the activating medium and endogenic cell respiration have been established. Cell respiration and phenol concentration in the assay solution positively correlated at a phenol concentration range of 0.1–2.0 mg/L and were linearly dependent in the range of 0.1–1.0 mg/L. A Clark membrane electrode was the physiochemical transducer. The assay may be completed within 5 min. The cells oxidize phenol, pyrocatechol, mesityl oxide, aniline, and do not react with a number of xenobiotics, sugars, and alcohol. With the exception of aniline, most components found in waste waters from phenol production affect neither the assay process nor the ability of these cells to use phenol as exogenic respiratory substrate. The immobilized cells retained their ability to utilize phenol as an exogenic respiratory substrate for up to 1 mo.