Single-Round, Multiplexed Antibody Mimetic Design through mRNA Display†

By combining the high efficiency enrichment via our continuous flow magnetic separation (CFMS) technique with the analytical power of next-generation sequencing, we have enabled the generation of antibody mimetics with a single round of mRNA display. This approach eliminates iterative selection cycles, providing a path to fully automated ligand generation to meet the goal of a renewable, proteome-wide affinity reagent resource.