Sorption-catalytic determination of histamine and lysine after planar chromatography using surfactants

The effect of ionic surfactants on the separation characteristics of histamine and lysine in model mixtures has been studied by planar chromatography. It has been demonstrated that the impregnation of paper in paper chromatography and the modification of the mobile phase with solutions of sodium n-dodecyl sulfate in thin-layer chromatography improve the selectivity of separation. Use of n-dodecyl sulfate made possible an increase in selectivity and, in some cases, sensitivity of sorption-catalytic determination. A combination of planar chromatography with the subsequent sorption-catalytic determination directly on the support made us possible to develop methods for determining histamine and lysine in the concentration range 1 × 10−12−5 × 10−11 and 1 × 10−7−1 × 10−5 M, respectively. The developed methods have been used to determine histamine in human saliva and lysine in blood plasma and pharmaceutical products.