Involvement of Protein Kinase C-Related Anti-apoptosis Signaling in Radiation-Induced Apoptosis in Murine Thymic Lymphoma(3SBH5) Cells

Abstract Nakajima, T., Yukawa, O., Azuma, C., Ohyama, H., Wang, B., Kojima, S., Hayata, I. and Hama-Inaba, H. Involvement of Protein Kinase C-Related Anti-apoptosis Signaling in Radiation-Induced Apoptosis in Murine Thymic Lymphoma (3SBH5) Cells. Radiat. Res. 161, 528–534 (2004). Protein kinase C (PKC; also known as PRKC) is known to be an important participant in radiation-induced apoptosis. However, its role is not fully clarified. Using 3SBH5 cells, which are radiation-sensitive thymic lymphoma cells, the involvement and functions of PKC were assessed in radiation- induced apoptosis. PMA (phorbol 12-myristate 13-acetate), a PKC activator, inhibited the radiation-induced apoptosis in 3SBH5 cells. On the other hand, chelerythrine, a PKC inhibitor, potentiated apoptosis. In addition, Go6976, a classical PKC (cPKC) inhibitor, which specifically inhibits PKC (α and βI), also promoted apoptosis. Interestingly, post-treatment (20 min after irradiation) with Go6976 had no effect on the radiation-induced apopto...