Development of on low cholesterol milk

This study was designed to develop β-cyclodextrin(β-CD) and determine the optimum conditions of different factors(mixing temperature,mixing time and centrifugal speed) on cholesterol reduction from milk. By dual quadratic rotary regression orthogonal design experiment,the optimum parameters were mixing temperature60℃,mixing time 60min,centrifugal speed 12000r/min,and β-CD addition 2%(w/w). Under this condition,cholesterol removal rate was highest,the cholesterol content was 0.18mg/100g and below the detection limit and β-CD residue was very low.