[The effects of chitosan on different-derived fibroblasts].

Objective To investigate the effects of chitosan on the biological activities of the fibroblasts derived from different tissues. Methods The biological activities of the fibroblasts derived from different tissues were evaluated with a MTT method for fibroblast proliferation, photic and electronic microscope for morphologic and subcellular structure, 3H-proline uptake method for collagen secretion and ELISA box for the secretion of TGF-β 1,FGF-AB, and IL-8. Results This study showed that the chitosan inhabited the proliferation of the fibroblasts and the secretion of the TGF-β 1,FGF-AB and collagen of the fibroblasts with a dose-depended manner in the normal skin, hypertrophic scar and keloid groups, but it stimulated the IL-8. However, there were no significant differences among the three groups ( P 0.05). Conclusion The chitosan could inhibit the growth, proliferation, biosynthesis and secretion of the fibroblasts, and it may be used to treat different scars.