NATIONAL WORKSHOP ON CLINICAL RESEARCH AND REGULATORY AFFAIRS - AN UPDATE

5-lipoxygenase products of arachidonic acid metabolism also act as second messenger, downstream to protein kinase A and protein kinase C, and modulate the action of these kinases in primary afferent nociceptors to mediate their sensitization to the mechanical and chemical stimuli. 5 and 12 lipooxygenase products act synergistically with PGE 2 to produce mechanical hyperalgesia. [8] In the present study, there was a dosedependent increase in the analgesic activity of AKBA in acetic acid-induced writhing. In case of tail flick test there was no difference in the analgesic effect of 100 and 200 mg/kg dose of AKBA. AKBA showed antinociceptive activity as early as 30 min which was increased up to 60 min and after that the effect declined, as showed in Table 2. The effect of AKBA at 200 mg/ kg, p.o. was more pronounced in tail flick test rather than aceticacid–induced writhing as compared to nimesulide (2 mg/kg, p.o.). AKBA showed lesser duration of action.